L4650

Sigma

 

Lectin from Artocarpus integrifolia

peroxidase conjugate

Synonym:Artocarpus integrifolia agglutinin, Jacalin
MDL number:MFCD00162973

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Description

Biochem/physiol ActionsJacalin is extracted from Jack fruit seeds; a glycoprotein reported to bind human serum and secretory IgA.1 Jacalin is not blood group specific.2 The most effective inhibitor of hemagglutination is methyl α-D-galactopyranoside. The lectin is specific toward the Thomsen-Friedenreich (T) antigen, but has subtle differences in its combining site when compared to the site of Arachis hypogaea (peanut) agglutinin.3 Jacalin was also demonstrated to be a mitogen of T-lymphocytes.1
Preparation NotePrepared from Peroxidase (P 8375) using a modification of a published method1, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Unit DefinitionOne unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Physical formContains sodium citrate

Properties

formlyophilized powder
compositionProtein, ~75% modified Warburg-Christian
conjugateperoxidase conjugate
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

References

Cited Reference1. Sastry, M. V., Analysis of saccharide binding to Artocarpus integrifolia lectin reveals specific recognition of T-antigen (β-D-Gal-(1→3)-D-GalNAc). J. Biol. Chem. 261, 11726, (1986)
 2. Roque-Barreira, M.C., Campos-Neto, A., Jacalin: An IgA-binding Lectin. J. Immunol. 134, 1740, (1985)
 3. Ahmed, H., Chatterjee, B.P. J. Immunol. Berlin 5, 125, (1986)
referenceO'Sullivan, M. J., et al., A simple method for the preparation of enzyme-antibody conjugates. FEBS Lett. 95, 311-313, (1978) Abstract